Clinical characteristics and immunohistochemical analysis. Glut1 is a protein transporter that is involved in the active. Considering expression localization, cyclin d1 positive cells in odontogenic keratocysts and dentigerous cysts were frequently confined in parabasal layer, different from radicular cysts and glandular odontogenic cysts. The aim of the present study was an evaluation of kai1 expression in radicular cysts rc, follicular cysts fc, orthokeratinized keratocysts ookc, and parakeratinized keratocysts pokc.
Cyclin d1 is a protein required for progression through the g1 phase of the cell cycle. Kai1cd82 is a tumor suppressor gene with decreased gene expression being associated with increased invasive ability of oral squamous cell carcinoma and as hypothesized for various odontogenic cysts and tumors. It also highlights the reclassification of odontogenic keratocysts to keratocystic odontogenic tumours. Teeth develop from budlike invagination of ectodermal lining of primitive oral cavity epithelium.
Cyclin d1 is expressed in all adult human tissues with the exception of cells derived from bone marrow stem cell lines both lymphoid and myeloid. Ncam cd56 expression in keratinproducing odontogenic. The invasive growth of okc, an odontogenic cyst, and the cystic behavior of ua, an odontogenic tumor, might be related to state of expression of cyclin d1 and p16 in the epithelium of these lesions. The aim of the present study was to analyze the expression levels of cyclin d1 ccd1, a nuclear protein that plays a crucial role in cell cycle progression, in a series of keratinproducing odontogenic cysts. A total of 36 cystic lesions were examined, all of which were histologically benign. A total of 58 keratinproducing odontogenic cysts, diagnosed over ten years and classified\ud according to the who 2005 criteria, were. There are several signs that point to the tumour character of these lesions. International association of dental research south african division, xiiv scientific meeting, emperors palace, johannesburg. Odontogenic keratocyst because of its behavior, many oral pathologists now consider the okc an odontogenic tumor that has a cystic form 2005 classi. Immunohistochemical staining of cyclin d1 on formalinfixed, paraffinembedded tissue sections of. Sep 24, 2019 evaluation and comparison of odontogenic keratocysts and detigerous cysts immunoexpression and immunostaining intensities of ki67 antigen by assessing the whole extent of the epithelium all epithelium layers in combination and each layer individually.
It is,388 base pairs long, and translates into 295 amino acids. A research report submitted to the faculty of health sciences, university of the witwatersrand, johannesburg, in partial fulfilment of the requirements for the degree of. Findings showed higher expression of cyclin d1 in parabasal layer of odontogenic keratocyst and the entire cystic epithelium of glandular odontogenic cysts comparing to dentigerous. The aim of this study was to analyze the expression of p73 and tnfrelated apoptosisinducing ligand trail in odontogenic tumors and cysts, and to clarify changes in the expression of these proteins. Learn vocabulary, terms, and more with flashcards, games, and other study tools. Expression of cyclin d1 was higher in uas compared to keratocyts p 0. Immunohistochemical staining of cyclin d1 on formalinfixed, paraffinembedded tissue sections of odontogenic keratocysts n23. Together with its binding partners cyclin dependent kinase 4 and 6 cdk4 and cdk6, cyclin d1 forms active complexes that promote cell cycle progression by phosphorylating and inactivating the retinoblastoma protein rb.
Primary intraosseous carcinoma of the jaws pioscc might arise from odontogenic epithelium, more commonly from a previous odontogenic cyst. Considering expression localization, cyclin d1 positive cells in odontogenic keratocysts and dentigerous cysts were frequently confined in parabasal layer, diff erent from radicular cysts and glandular odontogenic cysts. Expression of the tumour suppressor gene p53 in odontogenic cysts. Mar 10, 2016 keratinproducing odontogenic cysts kpocs are a group of cystic lesions that are often aggressive, with high rates of recurrence and multifocality. Odontogenic keratocystskeratocystic odontogenic tumours. Ki67 and cyclin d1 in odontogenic keratocysts and orthokeratinised jaw cysts. Cyclin d1 expression in odontogenic cysts odontojenik. Kai1 has been detected in all normal human tissues tested thus far 1, 2. The aim of the present study was to analyze the expression levels of cyclin d1 ccd1, a nuclear\ud protein that plays a crucial role in cell cycle progression, in a series of keratinproducing odontogenic cysts. Odontogenic cysts, odontogenic tumors, fibroosseous, and giant cell lesions of the jaws joseph a regezi d. Clinical characteristics and immunohistochemical analysis of. Our results showed that in the presence of rhdkk1, e3330stimulated expression of osteoodontogenic differentiation related genes and proteins.
Cyst is defined as a pathological cavity filled with fluid which is solid semisolid or gaseous form which may or may not be lined by epithelium cyst can occur within bone or soft tissues they may be asymptomatic or associated with swelling and pain. It belongs to a separate family of membrane glycoproteins the transmembrane4 superfamily. An immunohistochemical marker study namrata n patil 1, vijay wadhwan 2, abhishek singh nayyar 3, minal chaudhary 4, santhosh d reddy 5, kv chalapathi 6 1 department of oral pathology and microbiology, saraswatidhanwantari dental college and hospital and postgraduate research institute, parbhani, india 2 department of oral pathology and. Kai1 protein expression in odontogenic cysts sciencedirect. Expression of proliferation markers ki67 and cyclin d1 in. Immunohistochemical comparison of cyclin d1 and p16 in.
Expression of cyclin d1 was observed in all 54 samples evaluated. The differences in carcinoembryonic antigen expression between the four studied lesions were statistically significant p jaws. Cyst is defined as a pathological cavity filled with fluid which is solid semisolid or gaseous form. Odontogenic cysts clinical features radiographic features differential diagnosis yasmin moidin 2008 batch al azhar dental college thodupuzha 2. Kcots are unique due to their histological features, clinical characteristics and biological behaviour among odontogenic cysts 79. Informacion del articulo differential expression of cyclin d1 in keratinproducing odontogenic cysts objetives. The orthokeratinizing odontogenic cyst is considered an unrelated entity without risk of recurrence or aggressive growth or association with nevoid basal cell carcinoma syndrome. Introduction classification types of odontogenic cysts conclusion reference 3. Immunohistochemical expression of ncam, using a monoclonal antibody, was determined in a series of 58 kpocs comprising 12 orthokeratinized. Malignant transformation of an odontogenic cyst in a period. The expression of cyclin d1 was significantly higher p 0. Sep 30, 2012 introduction classification types of odontogenic cysts conclusion reference 3.
Immunohistochemical expression of ncam, using a monoclonal antibody, was determined in a series of 58 kpocs comprising 12. Differential expression of cyclin d1 in keratinproducing odontogenic cysts. Feb 12, 2015 verasirera b, fornernavarro l, verasempere f. The cyclin d1 protooncogene is an important regulator of g1 to s phase progression in many different cell types. Eightyfive odontogenic cysts, 28 rc, 22 fc, and 35 okc 16 ookc, 19 pokc were selected. Theyarise as sequelae ofdental caries from the effects ofinflamma.
There were statistically significant differences between cyclin d1 expression in odontogenic keratocysts and radicular cysts p 0. Immunohistochemical staining of cyclin d1 on formalinfixed, paraffinembedded tissue sections of odontogenic keratocysts n. Immunohistochemical comparative study of the odontogenic. Keratinproducing odontogenic cysts kpocs are a group of cystic lesions that are often aggressive, with high rates of recurrence and multifocality. The difference was statistically significant p expression of cyclin d1 in parabasal layer of odontogenic keratocyst and the entire cystic epithelium of glandular odontogenic cysts comparing to dentigerous. Findings showed higher expression of cyclin d1 in parabasal layer of odontogenic keratocyst and the entire cystic epithelium of glandular odontogenic cysts comparing to dentigerous cysts and radicular cysts, implying the possible role of g1s cell cycle phase disturbances in the aggressiveness of odontogenic keratocyst and glandular odontogenic cyst. Odontogenic cyst are a group of jaw cysts that are formed from tissues involved in odontogenesis tooth development. Intrabony cysts are most common in the jaws, because the mandible and maxilla are the only bones with epithelial.
Oralsurgery 548 dentalupdate julyaugust 2015 odontogenic cysts an overview abstract. Evidence of a radiopaque internalstructure should be sought in these two lesions. An immunohistochemical study on bcl6 expression in odontogenic cysts. Evaluation of ki67 and cyclin d1 expression in odontogenic keratocysts and orthokeratinised jaw cysts. Because cystic sp aces within the jaws have special characteristics, they require a more precise definition. This article aims to discuss the clinical features, radiological assessment, histopathology and management of a variety of odontogenic cysts. An immunohistochemical study on bcl6 expression in. Forty cases of odontogenic cysts were examined for expression of p53 protein by a immunohistochemical technique and positive results of the p53 protein were demonstrated by nuclear staining. Cyclin d1 is a regulatory subunit of cyclin dependent kinases cdk4 and cdk6. Immunohistochemical expression of glucose transporter 1 in. Computerized evaluation of the immunoexpression of ki67. Ki67 immunoexpression was evaluated in 15 odontogenic keratocysts and 6 dentigerous cysts using automated methods and the aperio. Immunohistochemical staining of cyclin d1 on formalinfixed, paraffinembedded tissue sections of odontogenic keratocysts n23, dentigerous cysts n20, radicular cysts n20 and glandular odontogenic cysts n5 was performed by.
The aim of this case is to illustrate that the clinician should consider that an apparent benign dentigerous cyst can suffer malignant transformation and that all material removed from a patient must be evaluated histologically. Pdf differential expression of cyclin d1 in keratin. Ki67 immunoexpression was evaluated in 15 odontogenic keratocysts and 6 dentigerous cysts using automated methods and the. The human ccnd1 gene is located on the long arm of chromosome 11 band 11q. Jomr conservative treatment protocol for keratocystic. The latter feature figure 3 identification of pcnausingpcioin the basal andparabasalcells of thep53positive recurrent odontogenic keratocyst illustrated infig 2. Ncam cd56 expression in keratinproducing odontogenic cysts.
Calcifying odontogenic cysts are uncommon lesions of variable clinical behavior that account for approximately 0. Ogden, chisholm, kiddie, lane table i expression ofp53in cysts ofthe oral cavity cyst type examined positive negative radicular cyst 12 0 12 dentigerous cyst 12 0 12 odontogenic keratocyst 12 5 7 tocyst and dentigerous cyst are both devel opmental cysts, but radicular cysts are inflammatory in origin. Kpocs included orthokeratinised odontogenic cyst ooc and parakeratotic odontogenic cysts, which are now considered true tumours denominated keratocystic odontogenic tumours kcots. Odontogenic cysts, odontogenic tumors, fibroosseous, and. To assess p53 protein expression in a range of odontogenic cysts arising in the mouth, including those of developmental and inflammatory origin. Expression of p73 and trail in odontogenic cysts and tumors. Glut1 is a protein transporter that is involved in the. The present study was undertaken to compare the pattern of expression of egfr, cyclin d1, ki67, p53 and carcinoembryonic antigen cea in the epithelial lining of odontogenic keratocysts, dentigerous cysts. To investigate immunohistochemically the expression of neural cell adhesion molecule ncam, which has been identified as a signaling receptor with frequent reactivity in ameloblastomas ab, in a series of keratinproducing odontogenic cysts kpocs. Differential expression of cyclin d1 in keratinproducing odontogenic cysts article pdf available in medicina oral, patologia oral y cirugia bucal 201 december 2014 with 35 reads. Differential diagnosis ameloblastjc fibroma cystic ameloblastoma the internal structure in both of themdifferentiate adenomatoid odontogenic tumors calcified odontogenic cysts both can surround the crown androot of the involved tooth.
Evaluation and comparison of odontogenic keratocysts and detigerous cysts immunoexpression and immunostaining intensities of ki67 antigen by assessing the whole extent of the epithelium all epithelium layers in combination and each layer individually. During the g1 phase, it is synthesized rapidly and accumulates in the nucleus, and is degraded as the cell enters the s phase. An odontogenic cyst is a pathological, epitheliallined cavity containing fluid or semifluid which arises from the epithelial remnants of tooth formation. Although cysts are common in the jaws, most are radicular cysts of in. Malignant transformation of an odontogenic cyst in a. Odontogenic cysts are closed sacs, and have a distinct membrane derived from rests of odontogenic epithelium. Cysts and odontogenic neoplasms 160 jaw cysts general features of jaw cysts to physicians, the term cyst means, simply, a space. Forty cases of odontogenic cysts were examined for expression of p53 protein by a immunohistochemical technique and positive results. Expression of p53 was evaluated immunohistochemically in a retrospective study of formalinfixed, paraffinembedded tissue.
Jun 07, 20 odontogenic cysts are those which arise from the epithelium associated with the development of teeth. Th e diff erence was statistically significant p pdf available in medicina oral, patologia oral y cirugia bucal 201 december 2014 with 35 reads. Evaluation of ki67 and cyclin d1 expression in odontogenic. The 1992 world health organization who classification of odontogenic cysts is widely recognized and categorizes them as developmental or inflammatory in nature. The transmembrane4 superfamily of glycoproteins is a family of glycoproteins that presumably cross the cell membrane four times. Differential expression of cyclin d1 in keratinproducing. The source of epithelium is from the enamel organ, the reduced enamel epithelium, the cell rests of malassez or the remnants of the dental lamina. The expression of cyclin d1 was higher in uas compared to okcs although p16 showed similar expression in. Immunohistochemical analysis was performed on 21 ameloblastomas, 15 keratocystic odontogenic tumors and 15 dentigerous cysts.
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